Autoantibody Standardization Committee

Antinuclear Antibodies (ANA) Reference Standards Available

One of the goals of the IUIS Autoantibody Standardization Committee (ASC) is to help laboratories improve standardization and quality control of testing of anti-nuclear and related autoantibodies. As part of this effort, the ASC, in cooperation with the Centers for Disease Control (CDC) USA, is making reference standards available for autoantibody specificities through Plasma Services Group, Inc. (PSG). In addition to the same 17 reference reagents previously available from the CDC, new reference reagents will be generated and distributed by PSG. Two new reference sera are for anti-mitochondrial and anti-rods/rings autoantibodies - for details, please see our publication: Calise SJ et al. Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies. Clin Chem Lab Med. 2018, in press. Send reprint requests to or

The following ANA reference standards are available free of charge (subjected to shipping charges only) to all qualified academic or commercial laboratories. Availability is limited to 1 vial of each ANA reference standard per laboratory. It is strongly recommended that each laboratory use our ANA standards only to calibrate and establish their own internal reference standards that can be used on a daily basis.

The images/results shown on this webpage were obtained with 1:80 dilutions of standard sera on commercial HEp-2 ANA slides (INOVA Diagnostics, San Diego, CA), followed by staining with DyLight 488-conjugated Donkey anti-human IgG (γ-chain specific) antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA).

To request reagents/standards, please fill out the Reference Reagents Order Request Form and email the completed form to Please CC your request to Special requirements may apply to some countries and additional biological clearance documents must be provided to facilitate shipment.

Limit 1 vial of each sample per laboratory.

Visit for detailed descriptions and images of a variety of ANA patterns.