Autoantibody Standardization Committee

Antinuclear Antibodies (ANA) Reference Standards Available

The following CDC ANA reference standards (also known as IUIS ANA reference standards, including anti-DNA reference standards and anti-ENA reference standards) are available free of charge to all qualified clinical or commercial laboratories. Our overall goal is to help these laboratories improve quality controls in the testing of anti-nuclear antibodies and related autoantibodies. Availability is limited to 1 vial of each ANA reference standard per laboratory. It is strongly recommended that each laboratory use our ANA standards only to calibrate and establish their own internal reference standards that can be used on a daily basis.

The images/results shown on this webpage were obtained with 1:80 dilutions of standard sera on commercial HEp-2 ANA slides (INOVA Diagnostics, San Diego, CA), followed by staining with DyLight 488-conjugated Donkey anti-human IgG (γ-chain specific) antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA).

To request reagents, please fill out the Reference Reagents Order Request Form and email the completed form to Please CC your request to Special requirements may apply to some countries and additional biological clearance documents must be provided to facilitate shipment.

Limit 1 vial of each sample per laboratory.

Visit for detailed descriptions and images of a variety of ANA patterns.